| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.045599 |
| Chromosome: | chromosome 1 |
| Location: | 4024343 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g026500 | ASP3 | (1 of 2) 3.4.23.12 - Nepenthesin / Nepenthes aspartic proteinase; Pepsin-type aspartyl protease | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGCCCTGCTGCACCAGCTGCTAGGAGTGACGGGCGAGCAGGGGAGCGGG |
| Internal bar code: | CATGATAGGTAGAGGTTAAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 996 |
| LEAP-Seq percent confirming: | 97.561 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACTACCAGCCTGCGTAGGT |
| Suggested primer 2: | CAAAACCACAGGCAGCAGTC |