| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.045619 |
| Chromosome: | chromosome 12 |
| Location: | 2335946 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g508100 | (1 of 2) PTHR21580//PTHR21580:SF21 - SHIPPO-1-RELATED // O(6)-METHYLGUANINE-INDUCED APOPTOSIS 2 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTGAGCAGTCTTTGGCCGGTAGTCCGTGCCCGCCGGCCCGTTGTTGGC |
| Internal bar code: | ACTGCGTCTTAGCGGCTAAGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 956 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 27 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCCAACAGCCCCTGCATA |
| Suggested primer 2: | TGAAACGAGTGTCCTCCGTG |