| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.045636 |
| Chromosome: | chromosome 12 |
| Location: | 3948894 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g515450 | UBC22 | (1 of 2) K10573 - ubiquitin-conjugating enzyme E2 A (UBE2A, UBC2, RAD6A); E2 Ubiquitin conjugating enzyme | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGTGAGTGATCAAAACACGGAACTCCTCACGTGCTTGACTGGCATTGGC |
| Internal bar code: | CGGGAGCTGGGGCTGAAGGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 672 |
| LEAP-Seq percent confirming: | 3.57143 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 54 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACGCATATGAGCCACACG |
| Suggested primer 2: | AAAATGCACCTTGCCACGTC |