Insertion cassette: | CIB2 |
Side of cassette: | 3' truncated? |
Strand: | - |
Strain: | CLIP2.045638 |
Chromosome: | chromosome 2 |
Location: | 1200521 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g081550 | HAD | Putative 3-hydroxyacyl-ACP dehydratase (mitochondrial FAS); (1 of 1) K18532 - adenylate kinase (AK6, FAP7) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTAGGCACGCGCTTGGAAGCCTGAGACCCGTGTCCCCTACTCCCCATG |
Internal bar code: | TAAAGTGTCCCCCGTTACCGTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1152 |
LEAP-Seq percent confirming: | 97.6744 |
LEAP-Seq n confirming: | 42 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ACAGCTCCCCAGTCCAAATG |
Suggested primer 2: | TCACTGGACATGGGCTTCAC |