| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.045644 |
| Chromosome: | chromosome 3 |
| Location: | 275596 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g144404 | (1 of 14) PF03133 - Tubulin-tyrosine ligase family (TTL) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TACGACCCAAAGGCAGCACTGGAGTCCAACAGCAGTAGCAGTACCAGTAG |
| Internal bar code: | GACGAAGGTGGTATCGGCGTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 62 |
| LEAP-Seq percent confirming: | 33.3333 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACACGACAACACTTGGCTG |
| Suggested primer 2: | CAGCCAAGTGTTGTCGTGTG |