Insertion junction: CLIP2.045674_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.045674
Chromosome:chromosome 3
Location:6161189
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g190950 TUA1 (1 of 2) K07374 - tubulin alpha (TUBA); Alpha tubulin 1 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCTCGGAGACCGGTGCCGGCAAGCACGTGCCCCGCTGCATCTTCCTGG
Internal bar code:GACCAAGATTAGCACACTAGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2568
LEAP-Seq percent confirming:92.3077
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:GTGGACAGCACCGAGTTGTA
Suggested primer 2:ACCCAGCGTAGGAAAAGCTC