Insertion cassette: | CIB2 |
Side of cassette: | 5' |
Strand: | - |
Strain: | CLIP2.045675 |
Chromosome: | chromosome 7 |
Location: | 753813 |
Confidence (%): | 80 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre07.g317800 | (1 of 1) PF04091 - Exocyst complex subunit Sec15-like (Sec15) | intron |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGGGGATTCATCAACTGCCTCCTGCCCCGCTCGCGTCGCCGCAGGTGCG |
Internal bar code: | TTTCATCCTATGTCTTTAGTTC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 902 |
LEAP-Seq percent confirming: | 45.4545 |
LEAP-Seq n confirming: | 5 |
LEAP-Seq n nonconfirming: | 6 |
LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CCCGACCTCTCACCTCCTTA |
Suggested primer 2: | TGCTCCGAATCAACTTGGCT |