Insertion junction: CLIP2.045676_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.045676
Chromosome:chromosome 14
Location:923048
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g613400 POC16 Proteome of centriole protein 16; (1 of 1) PTHR13720//PTHR13720:SF27 - WD-40 REPEAT PROTEIN // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGTTATGAATGGAAATGAAGGTTTGTGGCAGGCGGGTGGCGGTGTCGGC
Internal bar code:ATTGTTTTGACGGCATCTGGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:767
LEAP-Seq percent confirming:80.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:CTGGAATCCCCCAACCTTCC
Suggested primer 2:TCAACTCCACACCATTCCCG