Insertion junction: CLIP2.045691_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.045691
Chromosome:chromosome 6
Location:3355725
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g800655 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GATTGATGGTCTGGATGACACCGCTGTCGCTGACCAGCCCCTCACCGGCC
Internal bar code:TGAGAGTCACGGGGACTCACCT

Confirmation - LEAP-Seq

LEAP-Seq distance:4492
LEAP-Seq percent confirming:94.9153
LEAP-Seq n confirming:112
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:118

Suggested primers for confirmation by PCR

Suggested primer 1:GCCATCAGCTCCTCCATCTC
Suggested primer 2:ACAAGCAGAAGATCGGCACA