Insertion junction: CLIP2.045702_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.045702
Chromosome:chromosome 7
Location:449870
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g315350 (1 of 14) PF00566 - Rab-GTPase-TBC domain (RabGAP-TBC) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTTTCAATTCGCGCTGGTATCTACAACCGCCCTCCCCTCACACACACGC
Internal bar code:GGGTTATCGTCTGCTTGTTCCG

Confirmation - LEAP-Seq

LEAP-Seq distance:281
LEAP-Seq percent confirming:16.6667
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:5
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GCTCCATACCCACACACACA
Suggested primer 2:TCCAGACAAAGACTCCACGC