Insertion junction: CLIP2.045715_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.045715
Chromosome:chromosome 2
Location:1774375
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g086600 (1 of 7) PF12680 - SnoaL-like domain (SnoaL_2) CDS
Cre02.g086650 SMC2,DIV35 (1 of 1) K06674 - structural maintenance of chromosome 2 (SMC2); Structural Maintenance of Chromosomes protein 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCTGACTCGTCGAGCACGCCAGCAGCGTCGCCTGTTGCTGTTGACTGCG
Internal bar code:TCGTGGCTTTCCCACGGTGCTA

Confirmation - LEAP-Seq

LEAP-Seq distance:1651
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TGCATACGACTTGAAGCCGT
Suggested primer 2:ATCCGCACCAGCATTCTTCA