Insertion junction: CLIP2.045728_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.045728
Chromosome:chromosome 3
Location:2701226
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g160953 (1 of 1) 3.1.27.1//3.6.3.44 - Ribonuclease T(2) / Ribonuclease T2 // Xenobiotic-transporting ATPase / Steroid-transporting ATPase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAATCGGCTGGCTTGAGGGTGGTGCGGGAGAGGCAGATGCGGACCTACG
Internal bar code:GATCCCGGGAAGACGTTTAGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:689
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:TGGAACAACTCCTCTCCCCA
Suggested primer 2:TGAGTAGGAGAGGAGGGCAC