| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.045733 |
| Chromosome: | chromosome 3 |
| Location: | 5092974 |
| Confidence (%): | 40 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTATTGACTCCTTACTGCCGTGTAGCGTTACAAACCGCCACGGCCCCA |
| Internal bar code: | GCCCCAAGGCGAGGGCGGGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4454 |
| LEAP-Seq percent confirming: | 1.16279 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 85 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGAAGGTTGGTACGCACTCG |
| Suggested primer 2: | GCGTATCCGGTGATGGGATT |