Insertion junction: CLIP2.045741_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.045741
Chromosome:chromosome 1
Location:8113253
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g065457 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGGTCCAGAACGTCGTCGGCGAGCTCCAAGTGTGGCTGCATTTTGGCGC
Internal bar code:TGTATACATTAGCTGGGTAGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:2719
LEAP-Seq percent confirming:99.2424
LEAP-Seq n confirming:131
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:132

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCTCCAGACCGATACGTC
Suggested primer 2:CCACCTCAACCAGTGATGCT