Insertion junction: CLIP2.045741_4

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.045741
Chromosome:chromosome 2
Location:7674003
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g147350 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATATCCAACTAATAAATCTTGAGAGCTAAGGCATCCACCCATGATTTCAT
Internal bar code:CAGTGGTCTAATCCTCAACATA

Confirmation - LEAP-Seq

LEAP-Seq distance:3605
LEAP-Seq percent confirming:98.5714
LEAP-Seq n confirming:69
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:70

Suggested primers for confirmation by PCR

Suggested primer 1:CTCGTGACGACTATGGAGCC
Suggested primer 2:CTAACGGCGCCAGAATAGGT