Insertion junction: CLIP2.045749_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.045749
Chromosome:chromosome 7
Location:2079170
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g326050 KIL8 Putative kinesin-like motor protein; (1 of 2) PTHR13140//PTHR13140:SF410 - MYOSIN // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGCAACCGTACATGGCTTGACAAACAAAAAACAAACGAATGTAATACA
Internal bar code:TCTAGTTTCACTTAACATGGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:878
LEAP-Seq percent confirming:8.16327
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:45
LEAP-Seq n unique pos:49

Suggested primers for confirmation by PCR

Suggested primer 1:GCGATGCGAATGTCCTTGAC
Suggested primer 2:GGGTTACCATTACCGCACCA