Insertion junction: CLIP2.045760_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.045760
Chromosome:chromosome 10
Location:1872289
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g431150 FAP256,ROC22,CEP104 Flagellar Associated Protein 256; (1 of 1) PTHR13371:SF0 - CENTROSOMAL PROTEIN OF 104 KDA intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCGTAAGGCAGCAGGTGTGAACACACAGGGTGCTCCGCGACTCGTGTTTG
Internal bar code:CAGCGTGCTAGGGGTCCCAAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1144
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CAGTGCTTTCACCTTGTGGC
Suggested primer 2:CCCACCTTGAGCATTTTGGC