| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.045769 |
| Chromosome: | chromosome 14 |
| Location: | 2657582 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g625600 | FHL10 | (1 of 2) PF02010 - REJ domain (REJ) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAGCGGCCTTGAGGGCTGCATCCGCAAACATCTGACGGGAGTCATCCGTG |
| Internal bar code: | TCAGTATTGATGGGTCAACTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2755 |
| LEAP-Seq percent confirming: | 94.1176 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGACGCTGTATTGCTGTGT |
| Suggested primer 2: | GCCATTTGCATACCAGCGAG |