| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.045789 |
| Chromosome: | chromosome 3 |
| Location: | 4883251 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g178500 | RIB43,RIB43a | (1 of 1) PTHR14517//PTHR14517:SF6 - RIB43A-RELATED // SUBFAMILY NOT NAMED; Flagellar protofilament ribbon protein 43 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTTGACGGAGAGGACCTCACAGTGAGTGACATTCAGGGCATAAGGACA |
| Internal bar code: | GTTAGTCGACGTTGAGTAGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 290 |
| LEAP-Seq percent confirming: | 46.1538 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTACTCCGCCATTCCCACC |
| Suggested primer 2: | ACGTTCAGGCTCTGACACAG |