Insertion junction: CLIP2.045792_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.045792
Chromosome:chromosome 1
Location:4286393
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g029000 COQ5D,UMM1,COQD2 (1 of 2) PTHR10108//PTHR10108:SF813 - METHYLTRANSFERASE // SUBFAMILY NOT NAMED; Ubiquinone/menaquinone biosynthesis methyltransferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGAGCGGCTGGGCAGTGAGGAGCGCTGCCGCGCGGCCATGGCGGCCGCG
Internal bar code:GGATAATCCGATCTGCTCGTTG

Confirmation - LEAP-Seq

LEAP-Seq distance:3985
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:AACCTCGCCACATAACCTGG
Suggested primer 2:CTTGGACGTGATCACCTGCT