| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.045798 |
| Chromosome: | chromosome 1 |
| Location: | 3821994 |
| Confidence (%): | 63 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g025000 | SSA12 | cilia-sensing, structure and/or assembly; (1 of 1) K16056 - calcium release-activated calcium channel protein 1 (ORAI1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGACCGCAACCGCACGGAGGTCATGGAGAAGACGGAGCAGCTCAAGGC |
| Internal bar code: | CAGGCGTCTTAATAGCAACATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1784 |
| LEAP-Seq percent confirming: | 10.5263 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGCATGAACTCAGCCTCCT |
| Suggested primer 2: | CAAAGTTCATGTGCGTGCCA |