| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.045809 |
| Chromosome: | chromosome 1 |
| Location: | 2911546 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g800057 | (1 of 1) K00587 - protein-S-isoprenylcysteine O-methyltransferase (ICMT, STE14) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACGGCGTGCGTAAGGCACCGCTGAGATGGCCGGAACTGGTGCGCTCCAT |
| Internal bar code: | TCTACTCTGTTTTTGTCCATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3334 |
| LEAP-Seq percent confirming: | 71.4286 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGGAGTTTTTGCTTGCGG |
| Suggested primer 2: | GATGGCATTTGGACCGCATC |