| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.045826 |
| Chromosome: | chromosome 8 |
| Location: | 2831880 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g373878 | RAA13 | (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase; putative protein kinase associated with psaA trans-splicing complex | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGGGGAGCGGCGGGGTCGGGCAGTCCGGTGCGGGCGATGGCTGGCCTG |
| Internal bar code: | TCACGCTCATTGTTAAAGTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 940 |
| LEAP-Seq percent confirming: | 55.5556 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGGAGACGACGACTGTTG |
| Suggested primer 2: | ACATGACTTCCGTGGTCACC |