Insertion junction: CLIP2.045838_3

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.045838
Chromosome:chromosome 16
Location:3513977
Confidence (%):40
Locus systematic id Locus common name Defline Feature
Cre16.g801940 (1 of 1) PF00078//PF00385//PF00665 - Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_1) // Chromo (CHRromatin Organisation MOdifier) domain (Chromo) // Integrase core domain (rve) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGTAAAGGAGAATCGGGACAAGAACTCCATCCAGCGTGCTTGACGTCTGG
Internal bar code:AGGTGAAGAGTTGCCGGTCATT

Confirmation - LEAP-Seq

LEAP-Seq distance:728
LEAP-Seq percent confirming:85.7143
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:TACCGTTTGTCTGGACACCG
Suggested primer 2:CAGTAGCGCCTCGAAATTGC