Insertion junction: CLIP2.045843_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.045843
Chromosome:chromosome 2
Location:8093139
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g144250 CYP769A1,CYP13 (1 of 1) 1.14.13.30 - Leukotriene-B(4) 20-monooxygenase / LTB(4) omega-hydroxylase; Cytochrome P450, CYP4 superfamily intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATCAACCCCACACCGCCCCACACGCTCTGCCACCCGCGCCACCCACCAC
Internal bar code:CAGGATGTCGAAAGCGCCCCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:884
LEAP-Seq percent confirming:45.6522
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:25
LEAP-Seq n unique pos:46

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTGGCTACGACTGTGTGT
Suggested primer 2:CGGGAGGTTACATGCATGCT