| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.045867 |
| Chromosome: | chromosome 5 |
| Location: | 2277243 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g238100 | PTK25 | (1 of 66) 2.7.12.1 - Dual-specificity kinase; Protein tyrosine kinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTAGAGTTCCCGTCTTGTGCGCCCCTGCAATGTCCCGTGGGTACTCTC |
| Internal bar code: | AATATGCGCATCGATGTGGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5310 |
| LEAP-Seq percent confirming: | 94.1176 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAACTGTTTTGGCCAGAGC |
| Suggested primer 2: | AGGCTATACCACAGTCCCGT |