Insertion junction: CLIP2.045871_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.045871
Chromosome:chromosome 13
Location:1640776
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g573600 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGAAACTTGATGGGCGCACTGACACACGCACGCCGTCCGCGCAACTGC
Internal bar code:TAGTGCATAGAGTGGGGCGCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:2954
LEAP-Seq percent confirming:88.2353
LEAP-Seq n confirming:15
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:17

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCCCGTTCTTGAAGTCCT
Suggested primer 2:ATCGGAACTGCAGCACATCA