| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.045886 |
| Chromosome: | chromosome 1 |
| Location: | 7608456 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g053300 | AIH1 | Agmatine iminohydrolase; (1 of 2) 3.5.3.12 - Agmatine deiminase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCAAAGGATTGCTTCAGCGGAAATGTAAGCAATGAGCAGTGCAATGGCCC |
| Internal bar code: | CAGTTCATCGAGTCTGTATTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 538 |
| LEAP-Seq percent confirming: | 71.4286 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGATGCTGTGTGAGTGGCT |
| Suggested primer 2: | TCAACGCAAGCAACTCAACG |