Insertion junction: CLIP2.045895_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.045895
Chromosome:chromosome 6
Location:2927730
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g273200 (1 of 1) K03762 - MFS transporter, MHS family, proline/betaine transporter (proP) outside_mRNA
Cre06.g273250 GPA2,GPAT2 Glycerol-3-phosphate acyltransferase; (1 of 1) K13506 - glycerol-3-phosphate O-acyltransferase 3/4 (GPAT3_4, AGPAT9, AGPAT6) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTACACCACCAGTACCTACTTTGTGACACAAACAGGTAGGTTTTCGCCTG
Internal bar code:GGTTCAAGGGAGTAGTGGGACG

Confirmation - LEAP-Seq

LEAP-Seq distance:3489
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:71
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:71

Suggested primers for confirmation by PCR

Suggested primer 1:ACTTGACCTGCTTCCGAGTG
Suggested primer 2:CAACACCAACCACATGGCTG