| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.045905 |
| Chromosome: | chromosome 4 |
| Location: | 1176034 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g215550 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCTCGCCCTGGCCCTCGCCGCCAGCCGTGTCGCTGTTGATAAAAAGGAA |
| Internal bar code: | GCTACAGGGGATAAGGGTTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1165 |
| LEAP-Seq percent confirming: | 72.8155 |
| LEAP-Seq n confirming: | 75 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 103 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGTTCAAGAGGAGGCCAAC |
| Suggested primer 2: | TATCTCCTCCCACCCACCAG |