Insertion junction: CLIP2.045910_3

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.045910
Chromosome:chromosome 13
Location:1627600
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g573500 BUG12,FAP95 Flagellar Associated Protein 95 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCTTGCTGGAATCCGGTGTCCAATCCTCATCATGTCGACCTTGCCCCC
Internal bar code:CTTCGAGATGTTCATTGGCCGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1719
LEAP-Seq percent confirming:96.0
LEAP-Seq n confirming:72
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:75

Suggested primers for confirmation by PCR

Suggested primer 1:AGACAAGGAAGTGGAAGGCG
Suggested primer 2:CGCACCCTCATCCTCAAACT