| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.045912 |
| Chromosome: | chromosome 10 |
| Location: | 926226 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g424250 | BUG13,FAP90 | Flagellar Associated Protein 90 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGTGTAGAAACCCTTCTTCTTCTCCTGGTTGGAGTCCTTGTAGCGAATG |
| Internal bar code: | AGAAGGTTTATTAGGGTAGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3497 |
| LEAP-Seq percent confirming: | 39.0 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 61 |
| LEAP-Seq n unique pos: | 100 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAAGGGTGTGTTCAGCAAG |
| Suggested primer 2: | CAATTGCGACGCTTCTAGCC |