Insertion junction: CLIP2.045917_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.045917
Chromosome:chromosome 1
Location:5080891
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g035450 (1 of 1) PTHR23291:SF36 - PROTEIN XBX-6, ISOFORM B intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAAGCTCCGCGCAACCAAGTGCGTAATCCAAAATCCCAGGAAAGCTCTG
Internal bar code:TCGATTGTTGAGCTGCCCAAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1001
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:AACTGAAAGCGTTCGCACAC
Suggested primer 2:GCTCTCCATGTACGGCTACC