| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.045922 |
| Chromosome: | chromosome 1 |
| Location: | 3139520 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g020050 | (1 of 4) IPR000533 - Tropomyosin | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAAGCGGAGTGAGGACAGCGGTTTGGGAGCGGGGTGCAGCTATCGTTCC |
| Internal bar code: | TTGACGAGAGACCGCTAGTCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3350 |
| LEAP-Seq percent confirming: | 63.4615 |
| LEAP-Seq n confirming: | 33 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCACGTCTCCCAGTTACC |
| Suggested primer 2: | CCTCGTTTTTCACCCGCTTG |