| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.045927 |
| Chromosome: | chromosome 9 |
| Location: | 398502 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre18.g749797 | FAP281 | Flagellar Associated Coiled-Coil Protein 281 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTGTTTTGGGGTGTAAGTGCGTAATCAAAGGCGGGAACTTGAGGCTGTG |
| Internal bar code: | CAACGTAGACCTTTCTAAGGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 128 |
| LEAP-Seq percent confirming: | 71.4286 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCAGCTCCTGTTTGTGTGTG |
| Suggested primer 2: | TATCCGTTCCAGGGACTCGT |