Insertion junction: CLIP2.045937_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.045937
Chromosome:chromosome 10
Location:1177650
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTTGACCGCTGGCATTATTGTGCATATTTTACATTATTCAGCGCAAATC
Internal bar code:TCTCCACCGCAACGGACCTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1861
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CGGCAGCAATAAGTTCGACG
Suggested primer 2:AAGCTAGAGAATCCGGCTGC