| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.045940 |
| Chromosome: | chromosome 6 |
| Location: | 840256 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g255600 | EZY24,EZY1,EZY1A | Copy A in the EZY1 tandem gene cluster | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCCCGCCAAGATGGACAACCTGGCTGCCATGGCGGCCGTGGTGGGCAC |
| Internal bar code: | ACTAATTCTTCTGCACGCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3349 |
| LEAP-Seq percent confirming: | 11.5385 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGAGACGTGTGTGTCACA |
| Suggested primer 2: | GTTTGGCCAGCAACTCAAGG |