| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.045951 |
| Chromosome: | chromosome 1 |
| Location: | 5113290 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g035700 | (1 of 781) IPR000104 - Antifreeze protein, type I | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGACGACCCAGATGCGCCCGAGTACAAGGAGGACCGCAAGCGCTTCGT |
| Internal bar code: | GATTCTGATAATATGCAGTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 54 |
| LEAP-Seq percent confirming: | 3.125 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCGGCTTTGTTCCATTTG |
| Suggested primer 2: | CGGAATGGCCCAGTTACCTT |