Insertion junction: CLIP2.045954_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.045954
Chromosome:chromosome 17
Location:4851255
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g734961 (1 of 1) PTHR15239:SF6 - NUCLEAR EXPORT MEDIATOR FACTOR NEMF CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACACTCAAGCTGCGGAAGCACTGTCGTACGCGCCGAGTGGAGTCGGTGC
Internal bar code:GGGTTGCGATCGCACATAATCA

Confirmation - LEAP-Seq

LEAP-Seq distance:333
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:ACCGTGCCACATACACACAT
Suggested primer 2:GGGGAGGGGAGGTTGTAGAT