Insertion junction: CLIP2.045957_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.045957
Chromosome:chromosome 5
Location:2147093
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g237000 MAP1D Methionine aminopeptidase; (1 of 1) PTHR10804:SF82 - METHIONINE AMINOPEPTIDASE 1D, MITOCHONDRIAL intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAAGGAAAGGACCGCCAGACCCATGCAAGAATCAACGGGCTCGAACCA
Internal bar code:ACTATTGAAAGGCCGTGCGCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:211
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:GCTCTGTTGCATTCGCTAGC
Suggested primer 2:GGCCCGTTTGGTCTACATGA