| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.045977 |
| Chromosome: | chromosome 12 |
| Location: | 2708070 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g505050 | (1 of 13) PF00646 - F-box domain (F-box) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCAGGGGTGAGCACTGCAATGCAGCCTGGCTTGGGGGGGACGACACTG |
| Internal bar code: | ATCCATAATGTTGCTCAGGGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1217 |
| LEAP-Seq percent confirming: | 65.9091 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAAACAGACCCGTGTGCTTG |
| Suggested primer 2: | GCCCTCGACTCTTCACACAA |