| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.045978 |
| Chromosome: | chromosome 1 |
| Location: | 783668 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g004200 | (1 of 44) IPR000048 - IQ motif, EF-hand binding site | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTTGGAGTGCAGGCTGACGCGGGAACTGACGCACATGCACCTATGTAT |
| Internal bar code: | CTATGACAGGGCGTAGAGTTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2714 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGGAGATTTTGCAGGAGCC |
| Suggested primer 2: | CAAGGTTCTGCGCGAAACAA |