Insertion junction: CLIP2.045986_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.045986
Chromosome:chromosome 3
Location:5839186
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g188100 (1 of 8) IPR001810//IPR006553 - F-box domain // Leucine-rich repeat, cysteine-containing subtype intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTCGGCTTCATTGGCCCCCGCCCCCGCCCCCATCGGTTCGCAGCACCTG
Internal bar code:CTTAAGGTCATCTTTCTTGGAG

Confirmation - LEAP-Seq

LEAP-Seq distance:580
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TACCAGCTCCTGCACCATTG
Suggested primer 2:ATACCTTCACTGCCAGCACC