| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.046000 |
| Chromosome: | chromosome 1 |
| Location: | 6718908 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g048000 | TPM1 | Tropomyosin 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACATAGGAGGCACAGCAATCAGCACTCCTGGGGACTGCCACACCACAACC |
| Internal bar code: | GCATTCCAATTAGTCATTGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3349 |
| LEAP-Seq percent confirming: | 94.5946 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATCGAGGGCCAGGAGGAAA |
| Suggested primer 2: | TATGGCGATTCCCGTACTGC |