| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.046022 |
| Chromosome: | chromosome 13 |
| Location: | 3348840 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g586450 | NAT31 | N-acetyltransferase; (1 of 5) PF13508 - Acetyltransferase (GNAT) domain (Acetyltransf_7) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCGTTGCTGGAAGGAGGAGGCAGGGCCGTGGGATGGGGCGAGGATTGC |
| Internal bar code: | TTAAGGTATTACTCTTTTAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2160 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGCGGTTGTTTCAAGCTC |
| Suggested primer 2: | CGATGCTTCACTCACATGCG |