| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.046028 |
| Chromosome: | chromosome 2 |
| Location: | 5657196 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g115800 | (1 of 3) PF01987 - Mitochondrial biogenesis AIM24 (AIM24) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTTCACGGGGCCCGGCACTGTGTTCGTGCAGACGCGCTCCATGCAGAA |
| Internal bar code: | TCGTCGGCGGTCAAATGATGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4221 |
| LEAP-Seq percent confirming: | 78.0488 |
| LEAP-Seq n confirming: | 64 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 82 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTCCGTCCACATTGGCAT |
| Suggested primer 2: | CTGCTCATCTGCTCCTACGG |