Insertion junction: CLIP2.046044_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.046044
Chromosome:chromosome 13
Location:3726162
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g588495 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACATCTCGGAGCGAGTCTCGAGCCACGCGTCCACGTGCATTTCTAGTCT
Internal bar code:GGCGAACGAGCTCCTTGGGAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1666
LEAP-Seq percent confirming:81.8182
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:TTGTGAGGGGTGAGATGCAC
Suggested primer 2:TTTGTGAGTTGCCCCAAGGT