| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.046054 |
| Chromosome: | chromosome 3 |
| Location: | 3874945 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g170550 | POC4 | Proteome of centriole protein 4 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCCTGGATCGCCCTCCTTGGTCACGCAGAGGTACCCGCTGCATGTTGC |
| Internal bar code: | GGTCGTTAAGGCCGTAGGAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4388 |
| LEAP-Seq percent confirming: | 92.5 |
| LEAP-Seq n confirming: | 74 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 80 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGTGACGTTCATGCCCTA |
| Suggested primer 2: | CGTACCGTGTCGTACTACCG |