Insertion junction: CLIP2.046061_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.046061
Chromosome:chromosome 2
Location:331147
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g075200 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGGGTGCCGCCGCAGCAGCCCAGCGCACACATCCAGCGTCAGGCGCAGC
Internal bar code:GTAAGTCCTGATGACTATGATA

Confirmation - LEAP-Seq

LEAP-Seq distance:2386
LEAP-Seq percent confirming:67.2727
LEAP-Seq n confirming:37
LEAP-Seq n nonconfirming:18
LEAP-Seq n unique pos:55

Suggested primers for confirmation by PCR

Suggested primer 1:CTGACACACACGACATTCGC
Suggested primer 2:TAGGTCGTGCTCGTACATGC