Insertion junction: CLIP2.046072_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.046072
Chromosome:chromosome 14
Location:702396
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g612500 (1 of 1) K05954 - protein farnesyltransferase subunit beta (FNTB) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGAATCCAGGACGCTGAGGAGATACATGACTTTTTGGTTCTGCATCGCGA
Internal bar code:GATCGGATAACTGGACATTTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1466
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:CCCAATGCCTCCCACTTGAT
Suggested primer 2:CGTGGTTCAAGTGTGCAAGG